Pacemaking Activity in Normal Rat Kidney Fibroblasts
نویسندگان
چکیده
Proefschrift ter verkrijging van de graad van doctor aan de Radboud Universiteit Nijmegen op gezag van de rector magnificus prof. mr. General Introduction 9 Calcium signaling in control of life and death 10 Release and uptake of calcium from subcellular organelles 11 Influx and efflux of calcium from the extracellular medium and the cytoplasm 13 Role of the membrane potential in cellular signaling 17 NRK fibroblasts: a model system to study growth-dependent calcium dynamics and membrane excitability 18 Aim and outline of thesis 22 Local induction of pacemaking activity in a monolayer of electrically coupled quiescent NRK fibroblasts 117 Chapter 6 General Discussion 147 Mechanisms of calcium entry in NRK fibroblasts 149 Channels enabling calcium entry in NRK fibroblasts 150 Growth stage dependent calcium entry 153 Release and uptake of calcium from subcellular organelles 154 Induction of pacemaking activity in a monolayer of coupled NRK cells 157 Electrophysiological characterization of pacemaking activity in density-arrested NRK monolayers 163 Future perspectives 171
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Local induction of pacemaking activity in a monolayer of electrically coupled quiescent NRK fibroblasts.
Cultures of normal rat kidney (NRK) fibroblasts may display spontaneous calcium action potentials which propagate throughout the cellular monolayer. Pacemaking activity of NRK cells was studied by patch clamp electrophysiology and vital calcium imaging, using a new experimental approach in which a ring was placed on the monolayer in order to physically separate pacemakers within or under the ri...
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